Can Low-dose Irradiation of Donor Hearts before Transplantation Inhibit Graft Vasculopathy?
10.4326/jjcvs.28.30
- VernacularTitle:移植前のドナー心に対する低量放射線照射は心移植後の冠動脈硬化病変を抑制するか？
- Author:
Bungo Shirasawa
;
Kimikazu Hamano
;
Hiroshi Ito
;
Hidenori Gohra
;
Tomoe Katho
;
Yoshihiko Fujimura
;
Kensuke Esato
- Publication Type:Journal Article
- Keywords:
DNA
- From:Japanese Journal of Cardiovascular Surgery
1999;28(1):30-33
- CountryJapan
- Language:Japanese
-
Abstract:
This experimental study was conducted to histopathologically determine whether the low-dose irradiation of donor hearts before transplantation can inhibit graft vasculopathy. Immediately after donor F 344 rat hearts were removed, they were treated with a single dose of radiation using 7.5Gy, 15Gy, or no radiation (control group). The F 344 hearts were transplanted into Lewis rats heterotopically, and cyclosporine A was injected intramuscularly for 20 days after transplantation in all groups. The hearts were harvested 90 days after transplantation, and examined for intimal thickening using elastica van Gieson staining. Severe intimal thickening was observed in both the irradiated groups, the percent intimal area of the coronary arteries was significantly increased in both these groups, to 34.3±12.9 in the 7.5Gy group and 37.0±8.9 in the 15Gy group, compared with 23.1±9.8 in the control group (p<0.01). In conclusion, these findings show that low-dose irradiation to donor hearts before transplantation does not inhibit graft vasculopathy.
