Molecular Description of Macroorchis spinulosus (Digenea: Nanophyetidae) Based on ITS1 Sequences.
10.3347/kjp.2016.54.1.109
- Author:
Eun Jeong WON
1
;
Deok Gyu KIM
;
Jaeeun CHO
;
Bong Kwang JUNG
;
Min Jae KIM
;
Yong Woon YUN
;
Jong Yil CHAI
;
Dong Wook RYANG
Author Information
1. Department of Laboratory Medicine, Chonnam National University Hospital, Gwangju 61469, Korea.
- Publication Type:Brief Communication
- Keywords:
Macroorchis spinulosus;
ITS1;
sequencing;
crayfish
- MeSH:
Animals;
DNA, Ribosomal Spacer/*genetics;
Metacercariae/classification/cytology/genetics/isolation & purification;
Mice;
Phylogeny;
RNA, Ribosomal, 18S/genetics;
Trematoda/*classification/cytology/*genetics/isolation & purification
- From:The Korean Journal of Parasitology
2016;54(1):109-112
- CountryRepublic of Korea
- Language:English
-
Abstract:
We performed a molecular genetic study on the sequences of 18S ribosomal RNA (ITS1 region) gene in 4-day-old adult worms of Macroorchis spinulosus recovered in mice experimentally infected with metacercariae from crayfish in Jeollanam-do Province, Korea. The metacercariae were round, 180 µm in average diameter, encysted with 2 layers of thick walls, but the stylet on the oral sucker was not clearly seen. The adult flukes were oval shape, and 760-820 µm long and 320-450 µm wide, with anterolateral location of 2 large testes. The phylogenetic tree based on ITS1 sequences of 6 M. spinulosus samples showed their distinguished position from other trematode species in GenBank. The most closely resembled group was Paragonimus spp. which also take crayfish or crabs as the second intermediate host. The present study is the first molecular characterization of M. spinulosus and provided a basis for further phylogenetic studies to compare with other trematode fauna in Korea.
