The Evaluation of Immunochromatographic Assay kit for Rapid Detection of Hepatitis B Surface Antigen.
- Author:
Hyeong Soon SHIN
;
Young Bong KIM
;
Jung Woo SHIN
;
Chang Kyu KIM
;
Wang Sik LEE
;
Han Kyeom KIM
;
Kwang soon SHIN
- Publication Type:Original Article
- MeSH:
Emergency Service, Hospital;
Hepatitis B Surface Antigens*;
Hepatitis B*;
Hepatitis*;
Humans;
Immunochromatography*;
Limit of Detection;
Sensitivity and Specificity
- From:Journal of the Korean Society of Virology
1997;27(2):137-141
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
We evaluated Immunochromatographic assay kit to screen HBsAg in human serum. When the reference HBsAg was applyed to ICA, HA and EIA kits, the limit of detection for HBsAg were found out to be 4, 2 and 0.25 ng/ml respectively. But ICA kit required 5 minutes to read the result whereas HA and EIA kit more than one hour. The sensitivity was 97% (29 of 30 samples) and the specificity 100% (45 samples) compared with conventional EIA. The ICA kit needs no instrument or machine to perform the test contrary to the conventional methods. Therefore, this rapid and sensitive ICA kit can be used for HBsAg-screening, especially in the emergency room and in the scene of the accident.
