A DIGE PROTEOMIC ANALYSIS FOR LOW-INTENSITY EXERCISE-TRAINED RAT SKELETAL MUSCLE
- VernacularTitle:低強度・長時間水泳運動トレーニングによりラット骨格筋で発現するタンパク質のプロテオミクス;2D-DIGE解析
- Author:
WATARU YAMAGUCHI
;
ERI FUJIMOTO
;
MITSURU HIGUCHI
;
IZUMI TABATA
- Publication Type:Journal Article
- Keywords:
swimming;
epitrochlearis;
proteomics;
low-intensity exercise training
- From:Japanese Journal of Physical Fitness and Sports Medicine
2011;60(5):511-518
- CountryJapan
- Language:Japanese
-
Abstract:
Background: Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. Purpose: Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). Methods: For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. Results: Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. Conclusion: The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsynα, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.
