Determination of the Platelet Activating Factor in Silicotic Patients and its Effect on Fibroblasts
10.1265/ehpm.5.134
- Author:
Qunwei ZHANG
;
Yiqun MO
;
Jinpin LOU
;
Xinqiang ZHU
;
Zhimin CHEN
;
Linyun HE
;
Huixian ZHONG
- Publication Type:Journal Article
- Keywords:
Platelet activating factor (PAF);
Fibroblasts;
Silicosis
- MeSH:
Platelet Activating Factor;
g <3>;
Pathogenesis;
/mL;
Effective
- From:Environmental Health and Preventive Medicine
2000;5(4):134-137
- CountryJapan
- Language:Japanese
-
Abstract:
Platelet-activation factor (PAF), one of the potent proinflammatory mediators, is produced from a large range of cells, including polymorphonuclear neutrophils, monocytes, and natural killer cells. To study the role of PAF in the pathogenesis of silicosis, we determined the PAF in silicotic patients and in healthy persons. The results showed that the concentration of PAF in the plasma of silicotic patients was significantly higher than that of healthy persons. Our in vitro experimental results showed that the total numbers of fibroblasts were markedly raised with added PAF from 0 to 1 μ g/ml. Adding 1 μ g/ml PAF significantly increased the total numbers of fibroblasts after culture for 48, 72, 96 hrs. Therefore, we suggest that PAF be possibly involved in the pathogenesis of silicosis. However, the mechanism remains to be further elucidated.
