Effect of the mixed liquor of danshen and magnesium sulfate injection on IgG and IgM in serum of rabbits with lumbar intervertebral disc herniation.
- Author:
Zhi-bin LI
1
;
Pu-wei YUAN
;
Chao ZHU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drug Therapy, Combination; Drugs, Chinese Herbal; administration & dosage; Female; Humans; Immunoglobulin G; blood; Immunoglobulin M; blood; Injections; Intervertebral Disc Displacement; blood; drug therapy; immunology; Magnesium Sulfate; administration & dosage; Male; Phenanthrolines; administration & dosage; Rabbits; Random Allocation; Salvia miltiorrhiza; chemistry
- From: China Journal of Orthopaedics and Traumatology 2009;22(10):773-775
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of the mixed liquor of danshen and magnesium sulfate injection on inflammatory reaction caused by autoimmune response of rabbits with lumbar intervertebral disc herniation.
METHODSSixty rabbits were divided into blank group (10 rabbits), sham operation group (10 rabbits), model group (40 rabbits) according to method of random digits table. Then model group was divided into made group (group A, 10 rabbits), normal saline group (group B, 10 rabbits), aescin natrium group (group C, 10 rabbits) and danshen and magnesium sulfate injection group (group D,10 rabbits). After model success of lumbar intervertebral disc herniation, different drugs were given to rabbits with lumbar intervertebral disc herniation by ear margin vein. The rabbits of the group C,B,D were respectively given aescin natriu (0.5 mg/kg), normal saline (5 ml/kg), danshen and magnesium sulfate injection (2.0 mg/kg). The rabbits of blank group, sham operation group and group A were not given any disposal. The IgG and IgM level in serum of different groups were determined with ELISA method in fourteen day after model success and drugs given.
RESULTSThe level of IgG and IgM in the group A was higher than that of blank group and sham operation group (P < 0.05). The level of IgG and IgM in the group D was lower than that of group B,C (P < 0.05).
CONCLUSIONThe mixed liquor of danshen () and magnesium sulfate injection could inhibit inflammatory reaction caused by autoimmune response of lumbar intervertebral disc herniation,which provides a new alternative for lumbar intervertebral disc herniation.
