A study of the abnormalities of human epiderm in keloids and hypertrophic scars.
- Author:
Xiao-jie HE
1
;
Chun-mao HAN
;
Jia-ping PENG
Author Information
- Publication Type:Journal Article
- MeSH: Cicatrix, Hypertrophic; metabolism; pathology; Epidermis; metabolism; pathology; Female; Humans; Hyperplasia; Immunohistochemistry; In Situ Hybridization; Keloid; metabolism; pathology; Keratins; genetics; metabolism; Ki-67 Antigen; genetics; metabolism; Male; Tenascin; genetics; metabolism
- From: Chinese Journal of Surgery 2004;42(14):845-848
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the abnormalities of human epiderm in keloids and hypertrophic scars.
METHODSBiopsies from ten untreated keloids (duration of disease 3 - 30 years) and ten hypertrophic scars (duration of disease 6 - 10 months) and five normal adult skin specimens. Total RNA was isolated from normal adult skin. A cDNA fragment (base 5941 - 6481 bp) of the full-length human Tenascin-C cDNA was synthesized by polymerase chain reaction and subcloned in pGEM-T-easy. Dioxigen-labeled anti-sense and sense probes were synthesized by using a Sp6/T7 in vitro RNA synthesis kit in the present of Dig-UTP. In situ hybridization was performed on 4% paraformaldehyde-fixed and wax-embedded sections of keloids and hypertrophic scars. NBT-NCIP was used in color detection. Immunohistochemical procedure. The sections were incubated with antibodies (anti-Tenascin-C, anti-CK-16 and anti-Ki-67). Ultrasensitive Streptavidin Peroxidase staining was performed following established procedures.
RESULTSThe study show that the proliferation of epidermal keratinocytes in keloids and hypertrophic scars is very clear. The expressions of Tenascin-C mRNA in keloids epidermal keratinocytes markedly increased in contrast with epidermal keratinocytes of hypertrophic scars and adult skin. The CK-16 and Ki-67 stainings significantly enhanced in the epidermal keratinocytes of keloids and hypertrophic scars.
CONCLUSIONSThe different expressions of Tenascin-C, CK-16 and Ki-67 among normal adult skin, keloids and hypertrophic scars show the abnormalities of epidermal keratinocytes proliferation and differentiation in keloids and hypertrophic scars.