Reversal of multidrug resistance of MCF-7/ADR in nude mice by grape seed polyphenol.
- Author:
Cui-Juan ZHANG
1
;
Geng-Yin ZHOU
;
Li LI
;
Li-Li MA
;
Peng GAO
;
Hong LI
Author Information
- Publication Type:Journal Article
- MeSH: ATP-Binding Cassette, Sub-Family B, Member 1; metabolism; Animals; Antineoplastic Agents, Phytogenic; pharmacology; Apoptosis; drug effects; Breast Neoplasms; metabolism; pathology; Cell Line, Tumor; Doxorubicin; pharmacology; Drug Interactions; Drug Resistance, Multiple; drug effects; Drug Resistance, Neoplasm; drug effects; Female; Flavonoids; pharmacology; Humans; Mice; Mice, Nude; Neoplasm Seeding; Phenols; pharmacology; Polyphenols; Seeds; Vitis
- From: Chinese Journal of Surgery 2004;42(13):795-798
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the reversing effect of Grape seed polyphenol (GSP) on multidrug resistance of MCF-7/ADR cell in vivo.
METHODSThe transplantable breast carcinoma cell line MCF-7/ADR model was established in BALB/C-nu/nu mice by subcutaneous implantation. Flow cytometry (FCM) was used to investigate the changes of Pgp expression and apoptosis rate after different drug treatment.
RESULTSGSP has some effect on inhibition of tumor growth (the rate of inhibition was 18.35%), and combined with adriamycin can significantly inhibit tumor growth in nude mice, 20 mg/kg GSP can effectively reverse the resistance of MCF-7/ADR cells to ADR in vivo, and the rate of inhibition was 54.64%. FCM results showed that the expression of Pgp was significantly decreased (32.03 +/- 2.09) After administration of GSP and ADR, there was distinct difference between it and control (55.13 +/- 2.12). The mean rate of apoptosis was 15.12% +/- 1.04%, and was significantly increased compared with control (9.07% +/- 0.43%), P < 0.05.
CONCLUSIONGSP can effectively reversed the resistance of MCF-7/ADR cells in nude mice, the mechanism may be correlated with inhibition of Pgp expression and apoptosis.
