The significance of the expression of cyclooxygenase-2 in bladder transitional cell carcinoma tissues.
- Author:
Liang REN
1
;
Shu-kun HOU
;
Ling-feng HE
;
Hai-yun YE
;
Kao-peng GUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Blotting, Western; Carcinoma, Transitional Cell; enzymology; pathology; Cyclooxygenase 2; biosynthesis; genetics; Female; Humans; Male; Middle Aged; Neoplasm Staging; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Urinary Bladder; enzymology; Urinary Bladder Neoplasms; enzymology; pathology
- From: Chinese Journal of Surgery 2004;42(18):1104-1107
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of cyclooxygenase-2 (COX-2) in bladder transitional cell carcinoma tissues, and understand its clinical significance.
METHODSReversal transcription polymerase chain reaction (RT-PCR) was used to assess the expression of COX-2 mRNA in 52 cases of bladder transitional cell carcinoma tissues and 17 cases of normal bladder tissues far from neoplasm; Western blot was used to assess the expression of COX-2 protein in 49 cases of bladder cancerous tissues and 17 cases of normal tissues.
RESULTSPositive expression of COX-2 mRNA was detected in 83% (43/52) of bladder cancer tissues and in 29% (5/17) of normal tissues by RT-PCR and there was significant difference in expression of COX-2 mRNA between cancer tissues and normal tissues. Western blot analysis showed that expression of COX-2 protein was correlation with the stage and grade of cancer.
CONCLUSIONCOX-2 is overexpressed in bladder transitional cell carcinoma. COX-2 maybe play a certain role in carcinogenesis and progression of bladder cancer and turn into a useful target of chemoprevention of bladder cancer.
