The heat shock protein 70 antisense oligomers enhance the sensitivity of bladder cancer cell EJ to mitomycin C.
- Author:
Ling-feng HE
1
;
Shu-kun HOU
;
Zheng YAN
;
Liang REN
;
Shen-wu WANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Division; drug effects; Cell Line, Tumor; HSP70 Heat-Shock Proteins; antagonists & inhibitors; biosynthesis; genetics; Humans; Mitomycin; pharmacology; Oligonucleotides, Antisense; pharmacology; RNA, Messenger; biosynthesis; genetics; Urinary Bladder Neoplasms; genetics; metabolism; pathology
- From: Chinese Journal of Surgery 2004;42(18):1108-1110
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate whether the heat shock protein (HSP) 70 antisense oligomers can enhance the sensitivity of bladder cancer cell EJ to mitomycin C.
METHODSThe HSP70 mRNA of EJ cells was blocked by the 10 micromol/L HSP70 antisense oligomers, while its effect on cell growth was evaluated by methyl thiazolyl tetrazolium (MTT) and colony forming ability test.
RESULTSThe HSP70 expressions in HSP70 antisense treated group were lower than the corresponding sense and nonsense treated groups (P < 0.01). While, the increased sensitivity of EJ to mitomycin C was found in antisense treated group, compared with the corresponding sense and nonsense treated groups (P < 0.01).
CONCLUSIONThe sensitivity of bladder cancer cell EJ to mitomycin C was enhanced by the blockage of the HSP70 expression.
