Study on the interface of human hepatocyte L-02 polypropylene:simple culture method of human hepatocyte with spheroidal aggregate culture.
- Author:
Cheng-hong PENG
1
;
Bao-san HAN
;
Chang-you GAO
;
Zu-wei MA
;
Zhi-ming ZHAO
;
Yong WANG
;
Hong LIU
;
Gui-di ZHANG
;
Mei-juan YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Culture Techniques; methods; Cell Division; Cells, Cultured; Hepatocytes; cytology; Humans; Liver, Artificial; Membranes, Artificial; Polypropylenes; chemistry; Surface Properties; Tissue Engineering; methods
- From: Chinese Journal of Surgery 2004;42(17):1064-1068
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo found new interface of human hepatocyte/poly propylene with good cytocompatibility for made polypropylene hollow fibers bioreactor of bioartificial liver in future.
METHODSUsing the macromolecular hydroperoxide groups on the polypropylene membrane surface as initiators, acrylamides were polymerized on the polypropylene membranes, under induction by both UV irradiation and Fe2+ reduction. Growth characteristics of human hepatocyte L-02 were detected when it was cultured on polystyrene, polypropylene and modified polypropylene membrane surface.
RESULTSWater contact angle measurement of the polypropylene and the modified polypropylene membranes decreased from (72 +/- 5) degrees to (30 +/- 4) degrees , which indicated that the hydrophilicity of the membrane was improved obviously after the grafting modification. Human hepatocyte L-02 could not adhere and spread on modified polypropylene membrane surface, and grown in spheroidal aggregate with higher density and higher proliferation ratio measured by MTT method.
CONCLUSIONSAcrylamide polymerized on the polypropylene membranes is a good method which not only improved human hepatocytes cytocompatibility but also found a new simple culture method with spheroidal aggregate culture of human hepatocyte.