Expression and significance of caspase-3 gene in apoptotic muscle cells 103Pd radioactive stent bile duct in dogs.
- Author:
Gui-Jin HE
1
;
Qi SU
;
Qin-Yi GAO
;
Shu-He XU
;
Hong GAO
;
Wei-Guo JIANG
;
Tao JIANG
;
Xian-Wei DAI
;
Kai MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; radiation effects; Apoptosis Regulatory Proteins; Bile Ducts; enzymology; radiation effects; Caspase 3; Caspases; genetics; radiation effects; Dogs; Myocytes, Smooth Muscle; cytology; radiation effects; Palladium; administration & dosage; RNA, Messenger; genetics; radiation effects; Radioisotopes; administration & dosage; Reverse Transcriptase Polymerase Chain Reaction; Stents
- From: Chinese Journal of Surgery 2004;42(17):1069-1072
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo discuss the expression and significance of caspase-3 gene in the apoptotic muscle cells in gamma-radiation-induced muscle cell lines.
METHODSThe caspase-3 mRNA in the control and gamma-radiation induced apoptotic muscle cells was analysed by RT-PCR.
RESULTSThe expression of caspase-3 gene transcript was higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct, and apoptotic muscle cells were higher in 103Pd radioactive stent dog bile duct than in general stent dog bile duct.
CONCLUSIONSThe high level expression of caspase-3 gene may help to understand the muscle cells sensitivity to gamma-radiation apoptosis. 103Pd radioactive stent may increase the expression of caspase-3 gene in dog bile duct and prevent the billiary narrow when dog bile duct was injured by balloon.
