Effect of hepatitis B virus X gene on the expression of spastic paraplegia 21.
- Author:
Guo-sheng GAO
1
;
Peng-jian WENG
;
Reng-bin JI
;
De-zhou LI
;
Yong-yan LI
;
Hong-shan LI
;
Shi-xiong DING
;
Jing HU
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; genetics; metabolism; DNA, Viral; genetics; Hep G2 Cells; Hepatitis B virus; genetics; Humans; RNA, Messenger; genetics; Trans-Activators; genetics; Transfection
- From: Chinese Journal of Hepatology 2010;18(12):920-923
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of hepatitis B virus(HBV) X gene on the expression of SPG21.
METHODSThe expressions of SPG21 mRNA and protein in HepG2 and HepG2.2.15 cells were tested by RT-PCR and western blot. HepG2 cells were co-transfected with reporter plasmid pGL3-SPG21 and plasmids carrying individual genes of HBV, the luciferase activity was measured and the expressions of SPG21 were detected by RT-PCR and western blot.
RESULTSThe expressions of SPG21 mRNA and protein were higher in HepG2.2.15 cells than in HepG2 cells (0.36+/-0.06 vs 0.21+/-0.05, P value is less than 0.05). The activity of SPG21 in HepG2 cells transfected with pCMV-X was higher (875+/-27 vs 67+/-12, P value is less than 0.01) as compared to blank control group (transfected with pCMV-tag2B). HBV X gene enhanced SPG21 gene promoter activity, SPG21 mRNA expression and SPG21 protein production in HepG2 cells in a dose-dependent manner.
CONCLUSIONHBV X gene can specially activate SPG21 expression.
