Immunoassay for cadmium detection and quantification.
- Author:
Gong-Liang LIU
1
;
Ju-Fang WANG
;
Zhi-Yong LI
;
Shi-Zhong LIANG
;
Xiao-Ning WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; Cadmium; chemistry; Environmental Pollutants; chemistry; Food Contamination; analysis; Immunoassay; methods; Mice; Mice, Inbred BALB C; Reproducibility of Results; Sensitivity and Specificity
- From: Biomedical and Environmental Sciences 2009;22(3):188-193
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo detect cadmium in environmental and food samples by graphite furnace atomic absorption spectroscopy (GFAAS) and inductively coupled plasma atomic emission spectroscopy (ICPAES).
METHODSAn indirect competitive enzyme-linked immunosorbent assay (IC-ELISA) was developed based on a cadmium-specific monoclonal antibody. IC-ELISA for cadmium in environmental and food samples was evaluated.
RESULTSIC-ELISA showed an IC50 of 45.6 microg/L with a detection limit of 1.95 microg/L for cadmium, and showed a mean recovery ranging 97.67%-107.08%. The coefficient of variations for intra- and interassay was 3.41%-6.61% and 4.70%-9.21%, respectively. The correlation coefficient between IC-ELISA and GFAAS was 0.998.
CONCLUSIONIC-ELISA can detect and quantify cadmium residue in environmental or food samples.
