Rapid detection of rpoB gene mutations in rif-resistant M. tuberculosis isolates by oligonucleotide microarray.
- Author:
Ai-Hua SUN
1
;
Xing-Li FAN
;
Li-Wei LI
;
Li-Fang WANG
;
Wen-Ying ANS
;
Jie YAN
Author Information
- Publication Type:Journal Article
- MeSH: Antibiotics, Antitubercular; pharmacology; Bacterial Proteins; genetics; metabolism; DNA-Directed RNA Polymerases; Drug Resistance, Bacterial; genetics; Gene Expression Regulation, Bacterial; Mutation; Mycobacterium tuberculosis; drug effects; genetics; metabolism; Oligonucleotide Array Sequence Analysis; Rifampin; pharmacology
- From: Biomedical and Environmental Sciences 2009;22(3):253-258
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo detect the specific mutations in rpoB gene of Mycobacterium tuberculosis by oligonucleotide microarray.
METHODSFour wild-type and 8 mutant probes were used to detect rifampin resistant strains. Target DNA of M. tuberculosis was amplified by PCR, hybridized and scanned. Direct sequencing was performed to verify the results of oligonucleotide microarray.
RESULTSOf the 102 rifampin-resistant strains 98 (96.1%) had mutations in the rpoB genes.
CONCLUSIONOligonucleotide microarray with mutation-specific probes is a reliable and useful tool for the rapid and accurate diagnosis of rifampin resistance in M. tuberculosis isolates.
