Tetracysteine as a reporter for gene therapy.
- Author:
Chen-Yu XU
1
;
Ying GU
;
Wang-Heng HOU
;
Yu-Qiong QUE
;
Shuang-Guan GAO
;
Tong CHENG
;
Ning-Shao XIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; CHO Cells; Cricetinae; Cricetulus; Cysteine; analogs & derivatives; genetics; metabolism; Gene Expression Regulation; Genes, Reporter; Genetic Therapy; Humans; Lentivirus; genetics; Lymphocytes; metabolism
- From: Biomedical and Environmental Sciences 2009;22(6):496-501
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the feasibility of using tetracysteine (TC) reporter in gene therapy.
METHODSEffects of TC reporter and conventional reporter genes encoding green fluorescence protein (GFP) and luciferase (Luc) on expression and function of the therapeutic gene MGMT(P140K) were compared. Cytotoxicity and drug resistance were studied by Western blot. TC reporter used in therapy was analyzed by flow cytometry (FCM).
RESULTSThe TC reporter had no toxicity to cells and neither affected the expression or activity of therapeutic gene as compared to GFP and Luc. TC could be used in blood sample detection.
CONCLUSIONTC is a new kind of reporter gene for lentiviral vector in future gene therapy.
