Regulative effects of Chinese herbs for nourishing yin and removing fire on gene expressions of estrogen receptor alpha, insulin-like growth factor-1 receptor, epithelial growth factor receptor and protein synthesis in epiphyseal growth plate of female pubertal rats.
- Author:
Jun-ping LU
1
;
De-pei CAI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; Estrogen Receptor alpha; genetics; metabolism; Female; Gene Expression; drug effects; Growth Plate; drug effects; metabolism; Humans; Protein Biosynthesis; drug effects; Random Allocation; Rats; Rats, Sprague-Dawley; Receptor, Epidermal Growth Factor; genetics; metabolism; Receptor, IGF Type 1; genetics; metabolism; Yin-Yang
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(8):721-724
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Chinese herbs for nourishing yin and removing fire (NYRF) on gene expressions of estrogen receptor alpha (ER alpha), insulin-like growth factor-1 receptor (IGF-1R) and epithelial growth factor receptor (EGFR) in the epiphyseal growth plate of the female pubertal rats.
METHODSThe rats were randomly divided into the control group and the intervened group. Immunohistochemistry and realtime-PCR methods were used to measure the gene expression of ER alpha, IGF-1R and EGFR and their protein synthesis in epiphyseal growth plate.
RESULTSAfter being intervened with NYRF, the gene expressions of ER alpha and IGF-1R were down-regulated and their protein synthesis markedly reduced, while those of EGFR were unchanged.
CONCLUSIONNYRF can modulate the development and maturation of bone by regulating the expressions of ER alpha and IGF-1R in the epiphyseal growth plate.
