Effect of puerarin injection on the mRNA expressions of AT1 and ACE2 in spontaneous hypertension rats.
- Author:
Xu-ying YE
1
;
Hui SONG
;
Cheng-zhi LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Gene Expression; drug effects; Heart; drug effects; Humans; Hypertension; drug therapy; genetics; metabolism; Isoflavones; administration & dosage; Kidney; drug effects; metabolism; Male; Myocardium; metabolism; Peptidyl-Dipeptidase A; genetics; metabolism; RNA, Messenger; genetics; metabolism; Random Allocation; Rats; Rats, Inbred Dahl; Receptor, Angiotensin, Type 1; genetics; metabolism
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(9):824-827
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of puerarin on angiotensin II type 1 receptor (AT1) and angiotensin-converting enzyme 2 (ACE2) in spontaneous hypertension rat (SHR).
METHODSSHRs, 12 weeks old, were randomly divided into four groups: the model control group (A), the Verapamil group (B), and the two puerarin groups (C and D) treated by low dose and high dose of puerarin respectively. After being treated for 3 weeks, total RNA from tissues of heart, aorta and kidney in rats were extracted and mRNA expression levels of AT1 and ACE2 were determined by RT-PCR.
RESULTSAs compared with Group A, the mRNA expressions of AT1 and ACE2 in heart tissue were lower in Group C, and those in kidney tissue were higher in Group D (all P < 0.05); ACE2 mRNA expression was higher in Group D than in Group C (P < 0.05); no significant differences of the two indexes in aorta were shown among various groups. Besides, mRNA expressions of AT1 and ACE2 in heart and kidney tissue were proved to be positively linearly correlated.
CONCLUSIONHigh dose puerarin could increase the mRNA expressions of AT1 and ACE2 in kidney, while low dose puerarin could decrease them in heart; there might be a feed back correlation between AT1 and ACE2.
