Regulatory effect of procyanidins on the expressions of interferon-gamma, interleukin-12 and transcription factor T-bet mRNA in peripheral blood mononuclear cell of patients with alopecia areata.
- Author:
Pei-Cheng ZHU
1
;
Guo-Wei XUAN
;
Da-Can CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Alopecia Areata; drug therapy; genetics; immunology; Case-Control Studies; Cells, Cultured; Gene Expression; drug effects; Humans; Interferon-gamma; genetics; immunology; Interleukin-12; genetics; immunology; Leukocytes, Mononuclear; drug effects; immunology; Male; Middle Aged; Proanthocyanidins; administration & dosage; T-Box Domain Proteins; genetics; immunology; Young Adult
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(10):900-902
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulatory effect of procyanidins (PC) on the expressions of Th1 type cytokines (IFN-gamma and IL-12) and the transcription factor T-bet mRNA in peripheral blood mononuclear cell (PBMC) in patients with alopecia areata (AA).
METHODSAbove-mentioned expressions were detected using RT-PCR technique in 20 AA patients (8 of mild and 12 of severe degree) and 10 healthy subjects after phytoaemagglutinin (PHA) or PHA + PC stimulation.
RESULTSAfter being stimulated by PHA and PHA + PC, in patients with severe AA, the expression of T-bet mRNA was 0.581 +/- 0.148 and 0.419 +/- 0.113 respectively; that of IFN-gamma mRNA, 0.689 +/- 0.219 and 0.430 +/- 0.162; and that of IL-12 mRNA, 0.198 +/- 0.056 and 0.136 +/- 0.035, respectively. As compared with those before stimulation, the respective difference was statistically significant (P < 0.05).
CONCLUSIONSPC can inhibit the expressions of Th1 type cytokines and transcription factor T-bet in PBMC of severe AA patients, and reverse the Th1 responses. The authors offered that it maybe part of the acting mechanism of pine needle for treatment of AA.
