Effect of glycyrrhetinic acid on invasive capacity of leukemic cells and activity of gelatinase.
- Author:
Jin-Ling ZHANG
1
;
Xin-Yue LIU
;
Ping ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Cell Proliferation; drug effects; Down-Regulation; Gelatinases; metabolism; Glycyrrhetinic Acid; pharmacology; HL-60 Cells; Humans; K562 Cells; Leukemia; drug therapy; enzymology; pathology; physiopathology; Neoplasm Invasiveness
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(10):903-906
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of glycyrrhetinic acid (GA) on the invasive capacity of leukemic cells and the activity of intracellular gelatinase.
METHODSThe effect of GA, in different concentrations, on the proliferation of cultured K562 and HL-60 leukemic cells in vitro was determined by MTT assay; that on cell invasive capacity was tested by Transwell cubicle matrigel invasion assay; and that on the activity of gelatinase in cells was detected by gelatin zymography.
RESULTSGA showed significant inhibitory effect on the proliferation of K562 and HL-60 leukemic cells; it inhibited the invasive capacity of cells in concentration-dependent manner; and significantly down-regulated the activity of gelatinase A and B in cells.
CONCLUSIONSGA can inhibit invasive capacity of K562 and HL-60 leukemia cells by way of suppressing the activity of gelatinase A and B. This study provides an experimental evidence for preventing extra-medullary infiltration of leukemic cells.
