Effects of cryptotanshinone in lowering androgens synthesis for the prenatally androgenized male rats.
- Author:
Xi-He LI
1
;
Xin-Ming YANG
;
Xiao-Ke WU
Author Information
- Publication Type:Journal Article
- MeSH: Androgens; biosynthesis; Animals; Disease Models, Animal; Female; Humans; Male; Maternal Exposure; adverse effects; Phenanthrenes; administration & dosage; adverse effects; Polycystic Ovary Syndrome; drug therapy; metabolism; Pregnancy; Prenatal Exposure Delayed Effects; metabolism; Random Allocation; Rats; Rats, Wistar
- From: Chinese Journal of Integrated Traditional and Western Medicine 2008;28(11):1001-1004
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of cryptotanshinone on androgen synthesis for the prenatally androgenized male rats.
METHODSOn days 16-18 of pregnancy, rats were injected s. c. with testosterone propionas continuously for 3 days; male offspring were studied as subject. Serum concentrations of testosterone (T), 17a-hydroxy progesterone (17-OHP), blood glucose, and insulin were measured by radioimmunoassay. Then, the rats were treated with cryptotanshinone by gavage for 14 days, and the levels of serum T, 17-OHP and insulin were detected and the 17a-hydroxylase protein expression in interstitial cell was measured using the method of immunohistochemistry.
RESULTSThere was no difference between the male groups who were prenatally androgenized in serum levels of T, but the 17-OHP, fasting insulin levels and homeostatic model assessment for insulin resistance (HOMA-IR) elevated significantly (P < 0.05). Cryptotanshinone could lower the levels of 17-OHP (P < 0.05) but had no effect on 17a-hydroxylase.
CONCLUSIONPrenatally androgenized male rats exhibit elevated 17-OHP and diminished insulin sensitivity. Cryptotanshinone could decrease 17-OHP, but has no effect on insulin, indicating it may reduce androgen synthesis.
