Changes of protein kinases Mζ expression in the anterior cingulate cortex after applying three different magnitude of orthodontic force
10.3760/cma.j.issn.1002-0098.2014.12.011
- VernacularTitle:不同正畸力值作用下大鼠脑前扣带回皮质层蛋白激酶Mξ的表达
- Author:
Yu CHEN
1
;
Yinzi XIN
;
Chufeng LIU
;
Yijia CHEN
;
Yang CAO
Author Information
1. 中山大学光华口腔医学院·附属口腔医院正畸科·广东省口腔医学重点实验室
- Keywords:
Orthodontic extrusion;
Gyrus cinguli;
Cerebral cortex;
Protein kinases Mζ
- From:
Chinese Journal of Stomatology
2014;49(12):748-752
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the regulatory effect of central synaptic plasticity on pain induced by experimental tooth movement and to analyzethe expression of protein kinases Mζ(PKMζ) in the anterior cingulate cortex(ACC) after applying different magnitude of orthodontic force.Methods One hundred and thirty-six male Sprague-Dawley(SD) rats(200-250 g) were used in this study.Orthodontic tooth movement devices were placed on the teeth in the experimental group,and different orthodontic forces(0.39,0.78,1.17 N) were applied to move the maxillary first molars,respectively.The same mechanical devices were placed on the teeth in sham-treated group and no orthodontic force was applied.No orthodontic procedure was applied in blank control group.The average time spent on mouth-wiping behavior in each group was recorded after experimental tooth movement.Brain tissue of the anterior cingulate cortex was isolated on day 3 after experiment,and the expression level of PKMζ was analyzed with the method of immunohistochemistry and enzyme-linked immune sorbent assay.ζ-pseudosubstrate inhibitory peptide(ZIP),a selective inhibitor for PKMζ,was injected into ACC on day 3 after experimental tooth movement,and the effects of ZIP on mouth-wiping behavior were evaluated.Results No statistical difference was found between the blank control group and the sham-treated group in the average time spent on mouth-wiping,value of A and expression level of PKMζ(P＞0.05).Compared with the sham-treated group and blank control group,the average time of mouth-wiping behavior[(58.6±6.9),(66.3±7.8),(78.9±8.7) s],value of A(4 569±454,6 850±365,8 294±558) and expression level of PKMζ[(0.32±0.02),(0.34±0.02),(0.36±0.02) mg/L] in 0.39,0.78,1.17 N force group were found to be up-regulated with the increase of orthodontic force(P＜0.05).LSD test in three experimental sub-group showed statistical difference(P＜0.05).After microinjection of ZIP,the average time spent on mouth-wiping behavior significantly decreased(P＜0.01),while microinjecting saline did not change rats' mouth-wiping behavior(P＞0.05).Conclusions More pain caused by increased orthodontic force maybe due to the up-regulation of PKMζ in the anterior cingulate cortex.
