Effect of dentin proteoglycans on the bonding properties of dentin
10.3760/cma.j.issn.1002-0098.2014.12.012
- VernacularTitle:牙本质内源性蛋白聚糖对全酸蚀粘接剂即刻粘接性能的影响
- Author:
Yu GAO
1
;
Sanjun ZHAO
;
Peihuan WANG
;
Shuai LU
;
Xiaojing LI
;
Jihua CHEN
Author Information
1. 山东大学口腔医学院修复科·山东省口腔生物医学重点实验室
- Keywords:
Dentin;
Proteoglycans;
Dentin-bonding agents;
Tensile strength
- From:
Chinese Journal of Stomatology
2014;49(12):753-757
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential effect of proteoglycan(PG) and glycosaminoglycan(GAG) on the bonding of etch and rinse adhesive to dentin,in order to improve the bonding effect of dentin.Methods Forty-two extracted molars were used to obtain standard dentin bonding surface,and the specimens were etched for 15 s with 37％ phosphoric acid and divided into three groups using a table of random numbers.Then the three groups undergone different incubating procedures as follow:specimens in chondroitinase ABC(C-ABC) group were incubated with C-ABC at 37 ℃ for 48 h in vibrator.Specimens in trypsin(TRY) group were incubated with trypsin,and specimens in the control group were incubated with deionized water for 48 h in the oscillators.Then specimens in each group were randomly assigned into two subgroups,A(AdperTM Single Bond 2) and B(Prime & Bond NT) (n=7).The microtensile bond strength(μTBS),fracture mode and bonding interface morphology of the specimens were evaluated via microtensile testing,stereo microscope and field emission scanning electron microscopy(FE-SEM) respectively after specimens being incubated in 37 ℃ water for 24 h.Results The immediate μTBS of C-ABC group bonding with adhesive A and B [(32.9±2.5) and (26.8±2.2) MPa] were significantly lower than that of the control group [(40.7±3.3) and (34.6±3.7) MPa](P＜0.05).While the immediate μTBS of TRY group [(49.0± 3.6) and (44.5± 3.0) MPa] were significantly higher than that of the control group(P＜0.05).Conclusions Dentin PG participates in the dentin bonding process.Removal of PG increased the immediate μTBS of dentin and total etching adhesives,while removal of GAG decreased the immediate μTBS.
