Comparative study on contact guidance activity of human gingival fibroblasts on microgroove surfaces
10.3760/cma.j.issn.1002-0098.2015.01.008
- VernacularTitle:钛表面微沟槽形貌对人牙龈成纤维细胞接触诱导效应的比较研究
- Author:
Yingzhen LAI
1
;
Jiang CHEN
Author Information
1. 厦门医学高等专科学校口腔医学系
- Keywords:
Fibroblasts;
Titanium;
Microgroove;
Contact guidance
- From:
Chinese Journal of Stomatology
2015;50(1):33-37
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compared the difference incontact guidance activity among microgroove surfaces with different sizes of human gingival fibroblast(HGF),with the hope of providing basis for size selection of microgroove for transmucosal part of dental implant.Methods Basing on the size of HGF,microgroove titanium surfaces were fabricated by photolithography with parallel grooves:15,30 or 60 μm in width and 5 or 10 μm in depth.The groups that used different microgroove surfaces were denoted as T15/5,T15/10,T30/5,T30/10,T60/5,and T60/10.Group T0(the control meanwhile was a sputter of titanium on a simple planar silicon substrate).The morphology that HGF arranged along the groove was analyzed by immunofluorescence staining.Difference in contact guidance activity was quantitatively compared basing on the consistency of nucleus arrangement and deformation ratio.Results Microgroove groups had significantly higher consistency of nucleus arrangement and deformation ratio compared to the control group,with T60/10 had the highest consistency of 0.937±0.024,and T15/5 had the lowest consistency of 0.660± 0.016 and T60/10 had the highest deformation ratio of 3.555±0.205,and T15/5 had the lowest deformation ratio of 1.819±0.011.Conclusions Microgroove surfaces of all the different sizes show contact guidance activity on HGF,and the contact guidance activity increases with the increase of width and depth.
