The capacity and biological characteristic of the p75 neurotrophin receptor-positive ectomesenchymal stem cell in vitro.
- Author:
Manzhu ZHAO
1
;
Xiujie WEN
;
Gang LI
;
Kun YANG
;
Lin LI
;
Ming TANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers; metabolism; Cell Cycle; Cell Differentiation; Cell Division; Cell Movement; In Vitro Techniques; Mesenchymal Stromal Cells; chemistry; physiology; Rats; Rats, Sprague-Dawley; Receptor, Nerve Growth Factor
- From: Chinese Journal of Stomatology 2015;50(2):103-109
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75(+)EMSC and influencing factors.
METHODSThe immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process. Then EMSC was labeled by p75 neurotrophin receptor, and the cell cycle and stem cell surface antigens of the p75(+) EMSC was examined.
RESULTSImmunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days. The sorting rate of the p75(+)EMSC was 6.1%. The proportion of the p75(+)EMSCs' S/G2/M phase was stable during subculture. The special substances CD29, CD146 and Stro-1 were marked for p75(+)EMSC, and the expressions of the markers were all higher (> 90%) in p75(+) EMSC.
CONCLUSIONSThe embryonic tooth did not start to grow on the conception 12.5 days of SD rats. The p75(+) EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.
