The capacity and biological characteristic of the p75 neurotrophin receptor-positive ectomesenchymal stem cell in vitro
10.3760/cma.j.issn.1002-0098.2015.02.009
- VernacularTitle:p75神经营养受体阳性外胚间充质干细胞的体外获取与生物学特性研究
- Author:
Manzhu ZHAO
1
;
Xiujie WEN
;
Gang LI
;
Kun YANG
;
Lin LI
;
Ming TANG
Author Information
1. 400016,重庆医科大学附属第一医院口腔科
- Keywords:
Mesenchymal stem cell;
Receptors,nerve growth factor;
Organizers,embryonic;
Biological characterization
- From:
Chinese Journal of Stomatology
2015;50(2):103-109
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the biological characteristic of ectomesenchymal stem cells (EMSC) and the differentiation of p75+EMSC and influencing factors.Methods The immunohistochemical staining method was used to observe the migration of EMSC from 12.5 d SD rat embryonic facial process.Then EMSC was labeled by p75 neurotrophin receptor,and the cell cycle and stem cell surface antigens of the p75 + EMSC was examined.Results Immunohistochemical staining showed that stem cells from the cranial neural crest migrated to embryonic rat facial process at 12.5 days.The sorting rate of the p75+EMSC was 6.1％.The proportion of the p75 +EMSCs' S/G2/M phase was stable during subculture.The special substances CD29,CD146 and Stro-1 were marked for p75 +EMSC,and the expressions of the markers were all higher(＞90％) in p75+EMSC.Conclusions The embryonic tooth did not start to grow on the conception 12.5 days of SD rats.The p75 + EMSC after sorting had stable proliferation ability and had stem cell characteristics during subculture and didn't start differentiation.
