Effect of tamibarotene on innate immune response in the model of Porphyromonas gingivalis induced periodontitis
10.3760/cma.j.issn.1002-0098.2015.03.005
- VernacularTitle:他米巴罗汀对牙龈卟啉单胞菌诱导的牙周炎模型固有免疫应答的影响
- Author:
Ying JIN
1
;
Dixin LIU
;
Xu FANG
;
Kebei GAO
;
Xiaoping LIN
Author Information
1. 中国医科大学附属盛京医院口腔科
- Keywords:
Periodontitis;
Alveolar bone loss;
Tamibarotene;
Innate immune response
- From:
Chinese Journal of Stomatology
2015;50(3):146-150
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Am80 on innate immune response of Porphyromonas gingivalis(Pg) W83-induced periodontitis in mice.Methods Twenty-five mice were randomly divided into five groups,control group,PgW83-induced periodontitis group(periodontitis),Am80 (10 μg/d) treatment group(low-dose group),Am80(50 μg/d) treatment group(middle-dose group),Am80 (100 μg/d) treatment group(high-dose group).The distance of alveolar bone resorption in each mouse was observed and measured lby a dissecting microscope.Enzyme-linked immunosorbent assay(ELISA) was used to detect the level of serum anti-Pg specific IgG.The mRNA expression of interferon-γ(IFN-γ) and interleuking-12(IL-12) in gingival tissues,draining lymph node and spleen were detected bv real-time reverse transcriptase polymerase chain reaction(RT-PCR).The measurement data were statistically analvzed.Results The resorption rate in Am80 group[(121 ± 10)％] and high-dose of Am80 group[(108±8)％] was significantly different with that in periodontitis mice[(133± 10)％](P＜0.05).The serum levels of anti-Pg specific IgG of the Am80 groups of different doses(0.437 ± 0.083,0.566 ± 0.012,and 0.386± 0.078) were significantly lower than that of the periodontitis group(l.151±0.433) (P＜0.001).Real-time quantitative PCR assay showed that after Am80 treatment,the IL-12 mRNA levels in the gingival tissues,lymph nodes and spleen of mice were reduced to 1.107±0.088,0.806±0.220,and 0.668±0.756,which were all significantly different with those in periodontitis(P＜0.01).Similarly,the relative expression of IFN-γ mRNA levels in gingival tissue,lymph nodes and spleen of mice were reduced to 8.898±0.427,16.654±5.995,and 1.482± 0.033,which were significantly different with periodontitis(P＜0.001).Conclusions Am80 can reduce the extent of inflammation and alleviate alveolar bone resorption by modulating innate immune response.
