Effect of dopamine on the activity of matrix metalloproteinases and degradation of dentin collagen
10.3760/cma.j.issn.1002-0098.2015.03.015
- VernacularTitle:多巴胺对基质金属蛋白酶活性及牙本质胶原纤维降解的影响
- Author:
Qiangjian XU
1
;
Quanli LI
;
Jialong CHEN
;
Weibo ZHANG
;
Xiaoting WU
;
Ying CAO
Author Information
1. 安徽医科大学口腔医学院·口腔医院修复科安徽省口腔疾病研究中心实验室
- Keywords:
Dopamine;
Matrix metalloproteinases;
Dentin;
Collagen
- From:
Chinese Journal of Stomatology
2015;50(3):186-189
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibition effect of dopamine on the activity of matrix metalloproteinases(MMP) and the effect of dopamine on degradation of dentin collagen for its potential use in caries treatment and dentin adhesive.Methods In the experiment of MMP activity test,2.0 g/L dopamine+ 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the experimental group,and deionized water+ 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the negative control group,and 2％ chlorhexidine + 1.0 g/L highly purified collagenase type Ⅶ from Clostridium histolyticum served as the positive control group,and the mixture volume ratio of the two ingredients in every group was 1∶9.After 15 minutes,the enzyme activity of each sample was tested by MMP activity colerimetric quantitative detection kits,and the test was repeated 5 times in each group.In the experiment of collagen degradation,the dentin slices were demineralized with 37％ phosphoric acid for 1 min.In sequence,2 dentin slices were used to observe the morphology,and the remaining 30 dentine slices were randomly divided into three groups(n=10) according to random number table:the negative control ones were stored in 100 μl deionized water and 900 μl collagenase(7 days,37 ℃),the positive control ones were stored in 100 μl chlorhexidine and 900 μl collagenase(7 days,37 ℃) and the experimental specimens were stored in 100 μl dopamine and 900 μl collagenase(7 days,37 ℃).The degraded collagen was investigated by assaying hydroxyproline.The framework of collagen was evaluated with field emission scanning electron microscope(FE-SEM).Results The statistical results of completely random design ANOVA showed that the MMP activity and the amount of degraded collagen of the negative control group [(0.089±0.011) μmol · min-1· mg-1 and (2 837±201) μg/cm2] were significantly higher than those of the positive control group[(0.038± 0.006) μmol · min-1 · mg-1 and (1 288± 172) μg/cm2] and the experimental group[(0.030±0.009) μmol· min-1· mg-1 and (1 389±255) μg/cm2](P＜0.05).SEM observation indicated that the structural integrity of the collagen network on dentin still existed in experiment samples and positive control groups,however,collagen fibrils were destructed and the structural integrity disappeared in the negative control groups.Conclusions Dopamine may inhibit MMP activity and reduce the amount of degraded collagen.
