Point mutations and functions of mutated junctional adhesion molecule
- Author:
Pham Thien Ngoc
- Publication Type:Journal Article
- Keywords:
mutated junctional adhesion molecule
- MeSH:
Antigens;
mutation
- From:Journal of Vietnamese Medicine
2003;281(1):1-10
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
Using Quick change method, some pairs of primers were designed and 4 types of point mutation were created in the plasma past of JAM-1 endothelial cell adhesive molecule; C end acid amin was removed; JAM.1 Ser 284 amino acide was replaced by Ala acide amin, Tyr 261 amino acide by Phe amino acide; Tyr 261 and 280 were replaced at same time by 2 amino acided. The vectors containing mutant JAM was installed successfully in the cultured cell clones and promoting the biosynthesis of JAM-1 mutant proteins into cell clones. 4 types of mutant JAM-1 had changed HUVEC cells morphologically, leading to functional changes of protein
