Factor Xa Promotes Differentiation of Meg-01 Cell Line.
- Author:
Xiao-Lei YANG
1
;
Meng-Kai GE
2
;
Ai-Ping YU
3
;
Ying-Tao LYU
4
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Blood Platelets; cytology; drug effects; Cell Cycle Checkpoints; Cell Differentiation; drug effects; Cell Line; Cell Proliferation; Cell Survival; Factor Xa; pharmacology; Humans; MAP Kinase Signaling System; Megakaryocytes; cytology; drug effects; Phosphatidylinositol 3-Kinases; metabolism; Proto-Oncogene Proteins c-akt; metabolism
- From: Journal of Experimental Hematology 2016;24(2):519-525
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect and mechanism of Factor Xa on the differentiation of Meg-01 cells into platelet-like particles.
METHODSThe Meg-01 cells were used as experimental object, Factor Xa was used as agonist. Cell proliferation was detected by CCK-8 assay. The viability of platelet-like particles was analyzed by AlamaBlue kit. MAPK/ERK pathway and PI3K/AKT pathway were assayed by Western blot. The expression of CD41b was analyzed by Western blot and flow cytometry. Cell cycle and apoptosis were detected by flow cytometry.
RESULTSThe Factor Xa (1 µg/ml) inhibited cell viability, induced apoptosis. Factor Xa triggered cell arrest at the G(2)/M stage and down-regulated the expression of SKP2. After Meg-01 cells were stimulated by Factor Xa, the expression of CD41b was up-regulated and the MAPK/ERK pathway and PI3K/AKT pathway were activated. The platelets-like particles stimulated by FXa activation were viable.
CONCLUSIONThe Factor Xa maybe display some effect on the differentiation of megakaryocytes into platelets.
