Regulation of SIRT1 by SREBP-1 in Adipogenic Differentiation of Mesenchymal Stem Cells.
- Author:
Wen-Long HU
1
;
Jun CAO
2
;
Chang-Chang YIN
1
;
Ping-Ping WU
3
;
Chen YAN
1
;
Zi-Yao WANG
1
Author Information
- Publication Type:Journal Article
- MeSH: Adipocytes; cytology; Adipogenesis; Cell Differentiation; Cells, Cultured; Chromatin Immunoprecipitation; Gene Expression Regulation; Humans; Mesenchymal Stromal Cells; cytology; Promoter Regions, Genetic; Sirtuin 1; metabolism; Sterol Regulatory Element Binding Protein 1; metabolism; Up-Regulation
- From: Journal of Experimental Hematology 2016;24(2):590-595
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the regulation of SIRT1 by transcription factor SREBP-1 in adipogeneic differentiation of bone marrow mesenchymal stem cells (BMMSC).
METHODSOil red O staining was used to identify the adipogenic differentiation of BMMSC; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 gene were detected by RT-PCR; the expession level of SREBP-1 was determined by Western-blot. The chromatin immunoprecipitation (ChIP) assay was used to investigate the binding of SREBP-1 to SIRT1 promoter.
RESULTSBMMSC exposed to adipogenesis inducing medium become mature adipocytes at day 14; the mRNA transcription levels of AP2, LPL, SREBF-1, SIRT1 genes were up-regulated in adipocyte differentiation of BMMSC; the protein level of SREBP-1 was higher obviously; SIRT1 gene sequences was succesfully amplified from the genomic DNA immunoprecipitated by SREBP-1 antibody.
CONCLUSIONSREBP-1 can bind to the promoter region of the SIRT1 gene in adipogenesis of BMMSC, and may be involved in the transcriptional regulation of the SIRT1 gene.
