Polymorphism of Platelet Specific Antigens (HPA-1-5, 15) in Han Donors in the North Area of Henan Province.

Xue-lan Sun; Zhi-mei Yang; Jing-jing Zhang; Zhi-jun Yuan; Jun-ying LI; Gui-zhi Pang; Chen-guang Zhang

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Polymorphism of Platelet Specific Antigens (HPA-1-5, 15) in Han Donors in the North Area of Henan Province.

Author: Xue-Lan SUN ¹ ; Zhi-Mei YANG ¹ ; Jing-Jing ZHANG ¹ ; Zhi-Jun YUAN ² ; Jun-Ying LI ³ ; Gui-Zhi PANG ⁴ ; Chen-Guang ZHANG ⁵
Author Information

1. Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China.
2. General Hospital of Puyang Oil Field, Puyang 457001, Henan Province, China.
3. Henan Blood Center, Zhengzhou 45000, Henan Province, China.
4. Xinxiang Blood Center, Xinxiang 453000, Henan Province, China.
5. Henan Province Collaborative Innovation Center of Molecular Diagnosis and Laboratorial Medicine, School of Laboratory Medicine, Xinxiang Medical University, Xinxiang 453003, Henan Province, China. E-mail: 051085@xxmu.edu.cn.
Publication Type:Journal Article
MeSH: Alleles; Antigens, CD; genetics; Antigens, Human Platelet; genetics; Blood Platelets; China; DNA Primers; Ethnic Groups; GPI-Linked Proteins; genetics; Gene Frequency; Genotype; Humans; Neoplasm Proteins; genetics; Plateletpheresis; Polymerase Chain Reaction; Polymorphism, Genetic
From: Journal of Experimental Hematology 2016;24(2):602-606
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo study the gene polymorphism distribution characteristics of human platelet HPA-1-5 and 15 blood group antigens and construct a certain scale of platelet HPA database in the north area of Henan Province so as to provide platelet apheresis for clinical departments.
METHODSUsing polymerase chain reaction with sequence-specific primers (PCR-SSP), the genotyping of HPA-1-5 and 15 system was carried out; the periperal blood of 500 healthy Han donors in north area of Henan Province was collected randomly, the gene and genotype frequencies were detected by direct counting method, and the population distribution frequncy of HPA genes was analyzed by Hardy-Weinberg balance test, and compared with other regions and ethnics by using χ(2) test.
RESULTSThere was statistically significant (P < 0.05) of increase HPA-3b and HPA-5a in North area of Henan Province, compared with Chinese Han population; the HPA-3b and 5a increase and HPA-2a decrease were statistically significant (P < 0.05), compared with Ethnic minority of China. There was partly increase of HPA-1a, 2a, 3a and 5a, compared with different regions and ethnic in abroad. HPA allele genes of 500 Han donors in the North area of Henan Province were as follows: 0.985 and 0.015 for 1a and 1b; 0.924 and 0.076 for 2a and 2b; 0.469 and 0.531 for 3a and 3b; 1.000 and 1.000 for 4a and 5a; 0.532 and 0.468 for 15a and 15b, respectively. HPA allele gene frequencies were 1aa0.970, 1ab0.030; 2aa0.848, 2ab0.152; 3aa0.222, 3ab0.494, 3bb0.284; 4aa1.000; 5aa1.000; 15aa0.282, 15ab0.500, 15bb0.218. Compared with other regions and ethnic, HPA gene frequencies partly had statistical significance.
CONCLUSIONDistribution of HPA allele frequencies in the North area of Henan province is in accordence with the Hardy-Weinberg law. There are race and regional differences in HPA allele gene frequencies, compared with other regions and countries. And the HPA systems HPA-3 and 15 display the genetic polymorphisms, which provides a theoretical basis for the relevant research of the same type platelet infusion and alloimmune thrombocytopenia.