ADAR1 Knockout Inhibits Notch1-induced T-ALL in Mice.
- Author:
Hui-Er GAO
1
;
Lu-Yun PENG
2
;
Xin YANG
1
;
Ying-Chi ZHANG
1
;
Tian-Yuan HU
1
;
Jing XU
1
;
Wei-Ping YUAN
1
;
Tao CHENG
1
;
Ying-Dai GAO
3
Author Information
- Publication Type:Journal Article
- MeSH: Adenosine Deaminase; genetics; Animals; Disease Models, Animal; Mice; Mice, Knockout; Precursor T-Cell Lymphoblastic Leukemia-Lymphoma; genetics; Receptor, Notch1; genetics; T-Lymphocytes
- From: Journal of Experimental Hematology 2016;24(3):643-648
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of ADAR1 on the occurrence and development of mouse T cell acute lymphoblastic leukemia (T-ALL).
METHODSLck-Cre; ADAR1lox/lox mice were generated through interbreeding. The lineage-cells of Lck-Cre; ADAR1lox/lox mice and the control were enriched respectively by the means of MACS, and the lin- cells were transfected with retrovirus carrying MSCV-ICN1-IRES-GFP fusion gene. Then the transfection efficiency was detected by the means of FACS, and the same number of GFP+ cells were transplanted into lethally irradiated recipient mice to observe the survival of mice in 2 recipient group after transplantation.
RESULTST cell-specific knockout ADAR1 mice were generated, and Notch1-induced T-ALL mouse model was established successfully. The leukemia with T-ALL characteristics occured in the mice of control group, but did not in the ADAR1 kmockout mice after transplantation.
CONCLUSIONSADAR1 plays a key role in the incidence and development of Notch1-induced T-ALL.
