Effects of 17beta-estradiol on distribution of primordial germ cell migration in male chicks.
- Author:
Xiu-Mei JIN
1
;
Yi-Xiang ZHANG
;
Zan-Dong LI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Movement; drug effects; Chick Embryo; Estradiol; pharmacology; Female; Germ Cells; drug effects; Gonads; drug effects; Male
- From: Asian Journal of Andrology 2008;10(2):243-248
- CountryChina
- Language:English
-
Abstract:
AIMTo assess whether exogenous estradiol has any effect on migration of primordial germ cells (PGCs) in the chick.
METHODSFertilized eggs were treated with 17beta-estradiol (E(2)) (80 microg/egg) at stage X (day 0 of incubation), stages 8-10 (incubation 30 h) and 13-15 (incubation 55 h). Controls received vehicle (emulsion) only. Changes in PGC number were measured on different days according to developmental stages.
RESULTSIn male right gonads, but not in female left gonads, at stages 28-30 (incubation 132 h) significant decreases in the mean number of PGCs aggregating were observed compared with the controls (P < 0.05) while the total PGC number in the right and left gonads at each stage did not change (P > 0.05).
CONCLUSIONThe present study provides evidence that E(2) has significant effects on the localization of PGCs in male right, but not female left, gonads of chicken embryos at stages 28-30, compared with controls.
