Spatial and temporal expression of c-mos in mouse testis during postnatal development.
- Author:
Shao-Feng CAO
1
;
Ding LI
;
Qing YUAN
;
Xin GUAN
;
Chen XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fluorescent Antibody Technique, Indirect; Gene Expression; Genes, mos; genetics; Male; Mice; Reverse Transcriptase Polymerase Chain Reaction; Spermatocytes; growth & development; metabolism; Spermatogenesis; genetics; Testis; metabolism
- From: Asian Journal of Andrology 2008;10(2):277-285
- CountryChina
- Language:English
-
Abstract:
AIMTo immunolocalize the c-mos gene product and to investigate its spatial and temporal expression in mouse testis during postnatal development.
METHODSSemi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and in situ hybridization techniques were used to examine c-mos mRNA and indirect immunofluorescence was used to localize c-Mos protein in mouse testis on postnatal days 14, 21, 25, 28, 30, 35, 49 and 70.
RESULTSc-mos mRNA remained low on postnatal days 14-21, increased abruptly from day 25 and peaked on day 30. Its levels decreased a little on day 35 and became almost stable thereafter until day 70. c-mos mRNA was localized in the nucleus and cytoplasm of the spermatocytes and round spermatids. The nuclear staining was much stronger than the cytoplasmic staining. Using a polyclonal anti-c-Mos antibody, Western blotting detected a single band at 43 kDa in testis lysate. c-Mos protein was exclusively localized to the elongating spermatids and was first detected on postnatal day 30. The number of c-Mos-positive spermatids increased progressively till day 49 and stabilized thereafter.
CONCLUSIONThe c-mos gene displays a spatial and temporal expression pattern in the mouse testis during postnatal development at both the mRNA and protein level. This suggests that c-mos might play important roles in spermatogenesis.