Cytoprotective effects of Morinda officinalis against hydrogen peroxide-induced oxidative stress in Leydig TM3 cells.
- Author:
Mun-Seog CHANG
1
;
Won-Nam KIM
;
Woong-Mo YANG
;
Hyu-Young KIM
;
Ji-Hoon OH
;
Seong-Kyu PARK
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Catalase; metabolism; Cell Survival; drug effects; Cells, Cultured; Hydrogen Peroxide; adverse effects; Leydig Cells; cytology; drug effects; metabolism; Lipid Peroxidation; drug effects; Male; Mice; Morinda; Oxidative Stress; drug effects; Plant Extracts; pharmacology; RNA, Messenger; metabolism; Superoxide Dismutase; metabolism; Testosterone; metabolism
- From: Asian Journal of Andrology 2008;10(4):667-674
- CountryChina
- Language:English
-
Abstract:
AIMTo investigate the antioxidant effects of Morinda officinalis (Morindae radix, MR) on H(2)O(2)-induced oxidative stress in cultured mouse TM3 Leydig cells.
METHODSWe carried out 2,2-diphenyl-1-picrylhydrazyl free radical scavenging, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, lipid peroxidation, testosterone enzyme immunoassay, superoxide dismutase (SOD), and catalase (CAT) assays in Leydig TM3 cells.
RESULTSMR showed a 47.8% 2,2-diphenyl-1-picrylhydrazyl radical scavenging effect in TM3 cells with no significant cytotoxicity. Oxidative stress was induced in TM3 cells with 100 micromol H(2)O(2), and treatment of the cells with 250 microg/mL MR showed the most significant protective effect (64%, P < 0.001) in the cell viability assay with a decreased lipid peroxidation level (1.75 nmol/mg protein, P < 0.05), increased testosterone production (43.5 pg/mL), and improvements in SOD activity (7.49 units of SOD/mg protein, P < 0.001) and CAT activity (74.6 units of CAT/mg protein, P < 0.001).
CONCLUSIONThese findings indicate that MR, as an antioxidant, protects functions of cultured mouse TM3 Leydig cells from H(2)O(2)-induced oxidative stress.
