Detecting 8 common ? Thalassemia mutations in South East Asia by ASO method
- Author:
Tran Minh Hieu
- Publication Type:Journal Article
- Keywords:
Be ta, Thalassemia disease, mutation
- MeSH:
Mutation;
Thalassemia;
Diseases;
epidemiology
- From:Journal of Vietnamese Medicine
2003;280(1):1-5
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
An investagation was performed on 214 patients with the diagnosis of beta thalassemia in Ho Chi Minh City Hospital of Hematology and Blood Transfusion. Studying process composes of an extration of DNA and its multiplication in synthetic reaction chain of a 4 segment linking fractions and the detection of gene mutation with Bio-Rad’s kit of mD TM betha Gene. Results showed a demonstration of 8 mutations in the Rit of mDx, but some mutations such as CD95IVS1, CD19IVS5. The mutation type at the site of codon 26HbE (G-A) was at highest rate (31%) following with CD41-42, CD17, CD28. The application of the techniques of detection of various mutation has a necessary role in developing continues to improve the prevention of diseases.
