Fluoxetine Up-Regulates Bcl-xL Expression in Rat C6 Glioma Cells.
- Author:
Mi Ran CHOI
1
;
Dong Hoon OH
;
Seok Hyeon KIM
;
Byung Hwan YANG
;
Jun Seok LEE
;
Joonho CHOI
;
Hyun Soo JEON
;
Young Gyu CHAI
;
Yong Chon PARK
Author Information
1. Department of Neuropsychiatry, College of Medicine and Institute of Mental Health, Hanyang University, Seoul, Korea. hypyc@hanyang.ac.kr
- Publication Type:Original Article
- Keywords:
Antidepressant;
Bcl-xL;
Fluoxetine;
Galphai2;
Gsalpha;
Microarray
- MeSH:
Animals;
Apoptosis;
bcl-X Protein;
Cyclins;
Fluoxetine;
Gene Expression;
Glioma;
Rats;
RNA, Messenger
- From:Psychiatry Investigation
2011;8(2):161-168
- CountryRepublic of Korea
- Language:English
-
Abstract:
OBJECTIVE: To analyze both differentially expressed genes and the Bcl-xL protein expression after acute and chronic treatment with fluoxetine in rat C6 glioma cells. METHODS: C6 glioma cells were cultured for 24 h or 72 h after treatment with 10 microM fluoxetine, and gene expression patterns were observed using microarray and qRT-PCR. Then, cells were cultured for 6 h, 24 h, 72 h or 96 h after treatment with 10 microM fluoxetine, and the expression of Bcl-xL protein was measured using western blot. RESULTS: As determined by microarray, treatment with fluoxetine for 24 h up-regulated 33 genes (including Bcl-xL and NCAM140) and down-regulated 7 genes (including cyclin G-associated kinase). Treatment with fluoxetine for 72 h up-regulated 53 genes (including Gsalpha and Bcl-xL) and down-regulated 77 genes (including Galphai2 and annexin V). Based on the qRT-PCR results, there was an increase in Gsalpha mRNA and a decrease in Galphai2 mRNA at 72 h in fluoxetine-treated cells as compared to control, a result that was consistent with microarray. We also observed an increase in Bcl-xL mRNA (both at 24 h and at 72 h) in fluoxetine-treated cells as compared to control, demonstrating a tendency to increase gradually. Bcl-xL protein expression increased as the duration of fluoxetine treatment increased. CONCLUSION: These results suggest that chronic treatment with fluoxetine not only initiates the cAMP pathway through inducing Gsalpha expression but also induces Bcl-xL expression, thus inhibiting apoptosis.
