The expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro.
- Author:
Meng CAO
1
;
Yin DING
;
Lei SHU
;
Jia LI
;
Wei ZHANG
;
Tao GUO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Blotting, Western; Cells, Cultured; Estrogen Receptor alpha; genetics; metabolism; Estrogen Receptor beta; genetics; metabolism; Female; Fibroblasts; metabolism; Humans; Immunohistochemistry; Periodontal Ligament; cytology; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Stomatology 2007;42(9):533-536
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the expression of estrogen receptors in the human periodontal ligament fibroblasts in vitro.
METHODSHuman periodontal ligament fibroblasts were cultured in vitro. The estrogen receptors (ER)-alpha, ER-beta were detected with immunocytochemistry staining; The mRNA of ER-alpha, ER-beta were measured by reverse transcription polymerase chain reaction; The expression of ER-alpha, ER-beta protein were detected by Western blot.
RESULTSThe mRNA and protein expression of two subtype of ER were observed on human periodontal ligament fibroblasts (HPLF). The intensity of the ER-beta bands were stronger than those of ER-alpha, indicating that ER-beta expression in HPLF was higher.
CONCLUSIONSER may play an important role in the function of human periodontal ligament fibroblasts.
