Influence of iNOS silencing by RNA interference on proliferation activity of Tca8113 cell.
- Author:
Lan YANG
1
;
Wei-Liang CHEN
;
Shu-Guang ZENG
;
Shu-Biao ZHANG
;
Jing SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Squamous Cell; genetics; pathology; Cell Line, Tumor; Cell Proliferation; Gene Expression Regulation, Neoplastic; Genetic Vectors; Humans; Nitric Oxide Synthase Type II; genetics; metabolism; Plasmids; genetics; RNA Interference; Tongue Neoplasms; genetics; pathology; Transfection
- From: Chinese Journal of Stomatology 2007;42(9):547-549
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the apoptosis and proliferation activity of Tca8113 cells.
METHODSThe vector that involves short hairpin RNA of iNOS was transfected to Tca8113 cells. The change of iNOS expression was observed using immunohistochemistry technique, the apoptosis rate examined by flow cytometry, and the proliferation Tca8113 cells examined by methyl thiazolyl tetrazolium (MTT).
RESULTSThe expression of iNOS in Psilencer-iNOS group was lower than that in control groups (P < 0.01), the apoptosis rate was higher than that in control groups (P < 0.01); whereas the proliferation activity of Tca8113 cells in Psilencer-iNOS group was lower than that in control groups.
CONCLUSIONSDown expression of iNOS by RNAi can promotes apoptosis of Tca8113 cells and has an anti-proliferation activity effect.
