Proteomics study of the serum biomarkers in tongue cancer.
- Author:
Xin HUANG
1
;
Fei-Wu KANG
;
Long-Jiang LI
;
Yu-Ming WEN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Biomarkers, Tumor; blood; Carcinoma, Squamous Cell; blood; pathology; Cell Line, Tumor; Electrophoresis, Gel, Two-Dimensional; Humans; Mass Spectrometry; Mice; Mice, Inbred BALB C; Mice, Nude; Proteomics; methods; Tongue Neoplasms; blood; pathology; Xenograft Model Antitumor Assays
- From: Chinese Journal of Stomatology 2007;42(9):550-552
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.
METHODSThe tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).
RESULTSComparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice.
CONCLUSIONSThe squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.
