Expression, subcellular localization and nuclear translocation of transcription factor up stream stimulatory factor-1 in odontoblasts.
- Author:
Li-An WU
1
;
Ling-Ying WEN
;
Fu-Sheng YANG
;
Xiao-Jing WANG
;
Jun FANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; HeLa Cells; Humans; Nicotine; pharmacology; Odontoblasts; drug effects; metabolism; Protein Sorting Signals; Protein Transport; drug effects; Upstream Stimulatory Factors; metabolism
- From: Chinese Journal of Stomatology 2007;42(9):559-560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo examine the expression and subcellular localization of transcription factor USF1 in odontoblasts and investigate whether nuclear translocation occurs under stimuli.
METHODSOdontoblasts MDPC-23 were cultured on coverslips and divided into 2 groups. Group 1 received no stimuli, and group 2 was stimulated by nicotine with various concentrations respectively for 1h. Then the mountings of odontoblasts were prepared and immunocytochemical staining was performed with specific USF1 antibody via SABC method. Hela cells were used as positive control.
RESULTSThe staining was positive in the cytoplasm of odontoblasts in group 1, but in the nuclei of Hela cells and in 100 mg/L nicotine-stimulated odontoblasts in group 2.
CONCLUSIONSThere exists USF1 protein in odontoblasts, which locates in the cytoplasm and could translocate into nuclei under the stimulation of nicotine.
