Porphyromonas gingivalis vesicle induced cellular inflammatory responses of gingival epithelial cells in vitro.
- Author:
Yu-Rong KOU
1
;
Ya-Ping PAN
Author Information
- Publication Type:Journal Article
- MeSH: Bacterial Adhesion; Cells, Cultured; Cyclooxygenase 2; immunology; metabolism; Dinoprostone; immunology; metabolism; Epithelial Cells; immunology; metabolism; microbiology; Gingiva; immunology; metabolism; microbiology; Humans; Interleukin-6; immunology; metabolism; Interleukin-8; immunology; metabolism; Porphyromonas gingivalis; immunology; pathogenicity
- From: Chinese Journal of Stomatology 2007;42(9):564-567
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the model of cellular inflammatory responses of gingival epithelial cells in vitro induced by Porphyromonas gingivalis vesicle and to probe into the pathogenesis of Porphyromonas gingivalis in periodontitis.
METHODSThe effect of Porphyromonas gingivalis vesicle on prostaglandin E(2) (PGE(2)) production of gingival epithelial cells was detected by ELISA and the effects of Porphyromonas gingivalis vesicle on cyclooxygenase-2 (COX-2), interleukin (IL)-6 and IL-8 mRNA expression in gingival epithelial cells were determined by Real-time reverse transcription-polymerase chain reaction (RT-PCR).
RESULTSPorphyromonas gingivalis vesicle dose-dependently induced PGE(2) production and up-regulated COX-2, IL-6 and IL-8 mRNA expression in gingival epithelial cells significantly.
CONCLUSIONSCellular inflammatory responses of gingival epithelial cells induced by Porphyromonas gingivalis vesicle may contribute to the initiation and progression of periodontitis.
