Regulation of osteopontin expression in odontoblasts by upstream stimulatory factor 1.
- Author:
Li-An WU
1
;
Ling-Ying WEN
;
Fu-Sheng YANG
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Humans; Odontoblasts; metabolism; Osteopontin; genetics; metabolism; Plasmids; genetics; RNA, Messenger; genetics; Reverse Transcriptase Polymerase Chain Reaction; Transfection; Upstream Stimulatory Factors; genetics
- From: Chinese Journal of Stomatology 2007;42(11):663-664
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the regulation effects of upstream stimulatory factor 1 (USF1) on osteopontin expression in odontoblasts.
METHODSOdontoblast MDPC-23 was cultured and stably transfected with PCMV-USF1 or A-USF plasmids. Total RNA was extracted and osteopontin expression examined by semi-quantitative RT-PCR. Gray value of osteopontin was measured and statistic analysis performed.
RESULTSClones of stable PCMV-USF1 and A-USF plasmids transfection were obtained. Compared with the control, osteopontin was upregulated in PCMV-USF1 transfection group, and downregulated in A-USF transfection group.
CONCLUSIONSUpstream stimulatory factor 1 could regulate the osteopontin expression in odontoblasts, which could be blocked partly by A-USF.
