Establishment of an animal model of oral mucositis induced by conditioning regimen of haematopoietic stem cell transplantation.
- Author:
Xiao-Bing CHEN
1
;
Xuan WANG
;
Xiu-Zhen TONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Busulfan; toxicity; Cyclophosphamide; toxicity; Disease Models, Animal; Feasibility Studies; Hematopoietic Stem Cell Transplantation; Male; Mouth Mucosa; pathology; Rats; Rats, Sprague-Dawley; Stomatitis; chemically induced; etiology; pathology; Transplantation Conditioning; adverse effects
- From: Chinese Journal of Stomatology 2007;42(11):672-676
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rat model of oral mucositis (OM) induced by busulfan and cyclophosphamide (BUCY) conditioning regimen of hematopoietic stem cell transplantation (HSCT).
METHODSIn the model group, busulfan (6.0 mg.kg(-1).d(-1) x 4 d) and cyclophosphamide (120 mg.kg(-1).d(-1) x 2 d) were administered by intra-stomach perfusion and intraperitoneal injection, respectively. The left cheek mucosa were irritated by superficial scratching on day 6. The oral mucosal score (OMS) was assessed daily. Animals were sacrificed on day 7, 10, 13, 16 and 18. The samples of blood, bone marrow, and the oral mucosa were harvest to evaluate the clinical and histological changes.
RESULTSThe incidence of oral mucositis in model group was as high as 80.00% with a survival rate of 73.33%. The initial lesion on the oral mucosa was noted on day 7 with red spot and edema, and then progressive mucositis was characterized by large areas of ulcer formation. The duration of oral mucositis was 8 to 10 days. A continuous weight loss, white blood cell count decrease and bone marrow suppression occurred in the process of oral mucositis.
CONCLUSIONSAn animal model of conditioning regimen-induced oral mucositis was successfully established.
