Effect of fibrinogen on the secretion of interleukin-1β and-8 by polymorphonuclear leukocytes
10.3321/j.issn:1002-0098.2008.01.004
- VernacularTitle:纤维蛋白原对中性粒细胞分泌白细胞介素1β及8水平的影响
- Author:
Song GE
1
;
Ya-Fei WU
;
Tian-Jia LIU
;
Lei ZHAO
;
Shu MENG
Author Information
1. 遵义医学院附属口腔医院
- Keywords:
Fibrinogen;
Neutrophils;
Periodontitis
- From:
Chinese Journal of Stomatology
2008;43(1):8-11
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of one of the acute-phase proteins,fibrinogen,on the release of IL-1β and-8 by human peripheral polymorphonuclear leukocytes (PMN)and the possible role of fibrinogen during the destruction of periodontium. Methods Peripheral PMN were isolated by discontinuous density gradient centrifuging technique. The freshly isolated PMN were suspended in Hank's balaneed saline solution(1×109/L)supplemented with 0.5% BSA and 0.1% glucose.The levels of I-1β and-8 in the supernatants produced by cultured cells upon the addition of human fibrinogen at different concentrations were measured by ELISA technique. Results Incubated with human fibrinogen at 2 g/L or 10 g/L for different time periods,human peripheral PMN released significantly greater amount of IL-1β [(10.41±0.37)-(35.86±0.30)ng/L or(22.81 ± 0.45)-(57.77±2.08)ng/L]and IL-8[(93.90 ±13.95)-(2045.66±53.03)ng/L or(115.02±10.61)-(3858.69 ± 25.65)ng/L]than PMN without the stimulation offibrinogen(IL-1β,P＜0.001,and IL-8,P≤0.016).The higher concentration of fibrinogen or the longer treatment time,the higher levels of IL-1β and -8 were released by PMN(P＜0.001).Conclusions Fibrinogen induced the secretion of pro-inflammatory cytokines IL-1β and-8 by PMN and may be involved in magnification of the inflammatory response of periodontium and bone resorption.
