Biological transport of tetracycline hydrochloride by human periodontal ligament fibroblasts
10.3321/j.issn:1002-0098.2008.01.017
- VernacularTitle:人牙周膜成纤维细胞对四环素的跨膜转运
- Author:
Yu LIU
1
;
Hong-Chen LIU
;
Xia WU
;
Ling-Ling E
;
Bin LENG
Author Information
1. 海军总医院
- Keywords:
Fibroblasts;
Tetracycline;
Biological transport
- From:
Chinese Journal of Stomatology
2008;43(1):57-58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate biological transport of tetracycline hydrochloride by human periodontal ligament fibmblasts(HPDLF) for verifying the hypothesis of delivering medicine to the periodonfium and whole bodv through the root canal.Methods HPDLF and MC3,13-E1 cells were ineubated in antibiotics solutions. The intracellulaF antibiotics contents were measured by high performance liquid chromatography(HPLC) and the cell total protein was measured by bradford protein assay.Results The intracellular contents increased with ineubation time. The extracellular medicine concentration had effect on the intracellular contents. Conclusions Tetracycline hydrochloride can be transported into HPDLF with incubation and this transport is time-dependent and concentration-dependent.
