Quantitative Analysis of Immuno-fluorescence of Nuclear Factor-κB Activation.
- Author:
Min XIU
;
Feng HE
;
Yuanlei LOU
;
Lu XU
;
Xiong JIEQI
;
Ping WANG
;
Sisun LIU
;
Fei GUO
- Publication Type:Journal Article
- MeSH:
Active Transport, Cell Nucleus;
Cell Nucleus;
metabolism;
Cytoplasm;
metabolism;
Fluorescent Antibody Technique;
Human Umbilical Vein Endothelial Cells;
Humans;
NF-kappa B p50 Subunit;
metabolism;
Software
- From:
Journal of Biomedical Engineering
2015;32(3):669-674
- CountryChina
- Language:Chinese
-
Abstract:
Immuno-fluorescence technique can qualitatively determine certain nuclear translocation, of which NF-κB/ p65 implicates the activation of NF-κB signal pathways. Immuno-fluorescence analysis software with independent property rights is able to quantitatively analyze dynamic location of NF-κB/p65 by computing relative fluorescence units in nuclei and cytoplasm. We verified the quantitative analysis by Western Blot. When we applied the software to analysis of nuclear translocation in lipopolysaccharide (LPS) induced (0. 5 h, 1 h, 2 h, 4 h) primary human umbilical vein endothelial cells (HUVECs) , we found that nuclear translocation peak showed up at 2h as with calculated Western blot verification results, indicating that the inventive immuno-fluorescence analysis software can be applied to the quantitative analysis of immuno-fluorescence.
