Effects of Panicum miliaceum L. extract on adipogenic transcription factors and fatty acid accumulation in 3T3-L1 adipocytes.
- Author:
Mi Young PARK
1
;
Dong Won SEO
;
Jin Young LEE
;
Mi Kyung SUNG
;
Young Min LEE
;
Hwan Hee JANG
;
Hae Yeon CHOI
;
Jae Hyn KIM
;
Dong Sik PARK
Author Information
- Publication Type:Original Article
- Keywords: Cereals; anti-adipogenic; 3T3-L1 cell; differentiation; adipogenesis
- MeSH: 3T3-L1 Cells; Adipocytes; Adipogenesis; Cardiovascular Diseases; Edible Grain; Chronic Disease; Down-Regulation; Fatty Acids; Fatty Acids, Monounsaturated; Glycerol; Glycerophosphates; Incidence; Obesity; Oxidoreductases; Panicum; Peroxisomes; RNA, Messenger; Setaria Plant; Sorghum; Sterol Regulatory Element Binding Protein 1; Transcription Factors; Water
- From:Nutrition Research and Practice 2011;5(3):192-197
- CountryRepublic of Korea
- Language:English
- Abstract: The dietary intake of whole grains is known to reduce the incidence of chronic diseases such as obesity, diabetes, cardiovascular disease, and cancer. To investigate whether there are anti-adipogenic activities in various Korean cereals, we assessed water extracts of nine cereals. The results showed that treatment of 3T3-L1 adipocytes with Sorghum bicolor L. Moench, Setaria italica Beauvois, or Panicum miliaceum L. extract significantly inhibited adipocyte differentiation, as determined by measuring oil red-O staining, triglyceride accumulation, and glycerol 3-phosphate dehydrogenase activity. Among the nine cereals, P. miliaceum L. showed the highest anti-adipogenic activity. The effects of P. miliaceum L. on mRNA expression of peroxisome proliferator-activated receptor-gamma, sterol regulatory element-binding protein 1, and the CCAAT/enhancer binding protein-alpha were evaluated, revealing that the extract significantly decreased the expression of these genes in a dose-dependent manner. Moreover, P. miliaceum L. extract changed the ratio of monounsaturated fatty acids to saturated fatty acids in adipocytes, which is related to biological activity and cell characteristics. These results suggest that some cereals efficiently suppress adipogenesis in 3T3-L1 adipocytes. In particular, the effect of P. miliaceum L. on adipocyte differentiation is associated with the downregulation of adipogenic genes and fatty acid accumulation in adipocytes.
