Cytogenetic analysis of childhood acute lymphoblastic leukemia.
- Author:
Qing LIU
1
;
Hui JIANG
;
Heng-juan SUN
;
Yi-ju SONG
;
Li-ming BAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Chromosome Aberrations; Female; Fusion Proteins, bcr-abl; genetics; Humans; In Situ Hybridization, Fluorescence; Infant; Karyotyping; Male; Myeloid-Lymphoid Leukemia Protein; genetics; Precursor Cell Lymphoblastic Leukemia-Lymphoma; genetics
- From: Chinese Journal of Hematology 2012;33(4):282-285
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the genetic aberrations in pediatric acute lymphoblastic leukemia (ALL).
METHODSNinety ALL cases were enrolled in the study from January 2009 to November 2011. Chromosome banding analysis and fluorescence in situ hybridization (FISH) were used to detect genetic aberrations.
RESULTS(1) Chromosome analysis: 35 (53.0%) of 66 cases who had metaphase were abnormal, and 24 cases had no metaphase. (2) FISH analysis: among the 31 cases who had normal karyotypes and 24 who had no metaphase detected by chromosome banding technique, 7 (22.6%) and 14 (58.3%) cases were abnormal detected by FISH, respectively. There were no statistically significant differences compared with chromosome analysis (P = 0.655). Among these 55 ALL cases TEL/AML1, bcr-abl and MLL fusion genes were observed in 16 (29.1%), 3(5.5%) and 2(3.6%) cases, respectively. (3) Cytogenetic aberration was observed in 56 of total 90 ALL cases (62.2%).
CONCLUSIONSCytogenetic changes are common in childhood ALL. Conventional cytogenetic study could reliably detected chromosomal abnormalities for ALL with assessable metaphase. FISH should be used as a complementary method for ALL patients who have poor chromosomal morphology or no metaphase cells, and combination of both methods can improve the detection rate of genetic abnormalities in childhood leukemia.
